Acta Physica Polonica B

Vol. 34, No. 7, July 2003, page 3533


Gel Electrophoresis at High Fields

P. Pasciak, M.J. Krawczyk, M. Kopec, J. Dulak, K. Kulakowski

We investigate the band velocity v and the diffusion coefficient D of DNA in gel for the geometration mechanism. Here we treat the geometration as a one-step process. The velocity dispersion shows a maximum for some small number of hookings. This allows to expect that the diffusion constant behaves in a similar way. On the other hand, the average number of hookings should increase with the molecule length. Summarizing, the diffusion constant should decrease for very long molecules. However, we do not find this effect in experimental data. This contradiction can be resolved by a conclusion that multiple hookings do not occur.

PACS numbers: 05.60.Cd, 87.15.Vv


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